Dihydrofolate Reductase Inhibitory Peptides Screened from Structured Design -Loop Peptide Library Displayed on Phage
نویسندگان
چکیده
Experimental section General All chemicals and solvents for peptide synthesis were of reagent or HPLC grade and were used without further purification. TARON resin was purchased from Clontech. Dihydrofolate (DHP), nicotinamide adenine dinucleotide phosphate (NADPH) and methotrexate (MTX) were purchased from Wako Pure Chemical Industries. Biotin-AC5-OSu and 2-morpholinoethanesulfonic acid monohydrate (MES) were purchased from Dojindo. Dynabeads M-280 Streptavidin and Dynabeads M-280 Tosylactivated were purchased from Life Technologies. NeutrAvidin was purchased from Thermo Fisher Scientific. A -loop peptide phage library was produced according to previous our method.S1 RP-HPLC was performed on the Hitachi L7000 system using a COSMOSIL 5C18-ARII (10×250 mm) column for purification with a linear gradient of acetonitrile/0.1% trifluoroacetic acid (TFA) at a flow rate of 3.0 mL/min. ESI-MS was measured on a Shimadzu LCMS-2010.
منابع مشابه
Dihydrofolate reductase inhibitory peptides screened from a structured designed β-loop peptide library displayed on phage.
Enzyme inhibitory peptides with a loop structure stabilized using an antiparallel β-sheet scaffold (β-loop peptide) were obtained from a designed peptide phage library. Human dihydrofolate reductase (hDHFR) was used as the target enzyme. The obtained β-loop peptides were competitive inhibitors of hDHFR with micromolar inhibition constants and dissociation constants.
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